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For each biological replicates two technical replications were performed (n = 6).
The experiment was set up as a split plot design with four replicates, two main plots (irrigation treatments) and two sub-plots (cotton cultivars).
The evaluation consisted of a 2 × 2 factorial design with three replicates, two storage conditions (hermetic big-bags with and without CO2 injection, i.e., a modified atmosphere and a controlled atmosphere, respectively) and two sampling positions (upper and medium).
The experiment was designed as a split plot design with four replicates, two main plots (drip and sprinkler irrigation) and three sub-plots (vetch as green manure, red clover as green manure and control; without fertilization).
For each sampling event, two independent replicates (two tests at different time points) of the fish embryo test were conducted according to the work of Hallare and colleagues [12].
Experiments were performed in three biological replicates, two technical replicates and cDNA dilution series of 50 ng, 20 ng and 5 ng.
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Each test was replicated two times.
The experiment was replicated two times.
Temporal gene expression analysis was performed using six independent microarray hybridizations (three biological replicates × two dye-swap reactions) for each of six time points, with each slide containing two spots representing each gene at different array locations for a total of 12 signal intensity measurements per gene per time point.
Each group had three replicates with ten pigs per replicate.
Treatments were replicated three times.
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