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Tree reliabilities were tested with 1000 bootstrap replicates to yield a majority consensus tree.
We iterated this selection process in two independent replicates to yield 4 strains hereby named: ϕ6-Fast1, ϕ6-Fast2, ϕ6-Slow2, ϕ6-Slow2.
Under these circumstances, when a DSB-bearing chromatid replicates to yield a sister chromatid, the two sister chromatids will be unable to provide a useful repair template to one another.
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Each of the two strands of the parent DNA is replicated to yield the unchanged parent DNA and one newly synthesized DNA.
We combined the droplet counts (positive and negative) from all seven replicate wells to yield a single "metawell".
This procedure was repeated for β-actin in the 800 nm channel, and the protein signal was divided by the actin signal for each lane (replicate extraction) to yield the relative density.
Performance of the tree classification models could have been improved by computational approaches – such as collapsing the data into two classes (supersusceptible and "other"), replicating data to yield groups of equivalent numbers and combining data into a single training set.
The cross-validation procedure was replicated five times to yield 15 replicate datasets.
The remaining biological replicates were averaged to yield 424 samples.
The resulting 963 chips were normalized to the common range and the replicates were averaged to yield 424 samples.
Within each experiment the 20 replicates were averaged to yield a reliable signal for the respective probe.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com