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The low figure covered only in-work support during the introductory period of six months, while the high one covers in-work support, support for families with children where no-one is working and replicates support previously given to parents as a tax allowance.
Values in branches indicate the bootstrap (10,000 replicates) support values.
To test the stability of monophyletic groups in the ML trees, we calculated PHYML bootstrap (100 replicates) support values [ 108].
Very high correlations (r ≥ 0.95) between technical replicates support that the tag generation and barcoding process have not introduced any significant variability.
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Each treatment included three biological replicates (Supporting Information, Figure S1).
The significance level and the percentage of bootstrap replicates supporting each branch are indicated.
A fraction of the bootstrap replicates supports the alternative topology that unites Coleochaete with the Zygnematales (BP 12%and35%5%, respectively).
For an additional six outlier loci, the significant difference in allele frequency occurred in one of the treatment replicates (Supporting Information S1).
This was further illustrated by a hierarchical ANOVA of the relative transcript level data, where it was observed that 70 to 80% of the variation for the 'C-sources' and 'Stress' subsets took place at the level of the biological replicates, supporting the extremely low variation between genes as well as the low influence of conditions on the transcript levels.
In order to assess the alignment's tree like structure, likelihood mapping was carried out using Tree-Puzzle (version 5.2) [ 36], which generally showed alignments to have strong phylogenetic signals with 89-94% of quartets (1,000 pseudo-replicates) support a unique phylogeny (data not shown).
Internal consistency was high for all eight scales and the physical and mental health components underlying the scales were replicated, supporting the use of the standard scoring algorithms.
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CEO of Professional Science Editing for Scientists @ prosciediting.com