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Of note, the SEQC study [ 34] results showed a large degree of variation in sample sequencing library preparation even at the same site, but that the sequencing library replicates prepared at a single site and then sequenced at multiple laboratories resulted in very consistent measurements between sites.
Ground root materials (1 g) sampled from each of the replicates prepared from four S. flavescens population were extracted with 20 ml methanol (80 20, v/v in H2O) for 15 min, and the filtrate volume was re readjusted to 20 ml with extraction reagent.
Three independent replicates, prepared from independent biological samples, were analyzed.
To account for possible unrelated variations, the experiment involved biological replicates prepared on four occasions (see Materials & Methods section).
A precise, i.e., reproducible, test statistic should produce similar values for both subsets since all of the arrays in both subsets were drawn from a pool of replicates prepared from identical biological experiments.
After filtering, we discarded the two replicate arrays prepared in our laboratory and used only the replicates prepared at the BBC for final analyses, which thereby included 12 MS arrays and 15 control arrays.
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Three independent biological replicates were prepared and each replicate included root materials from three or more of P. ginseng plants.
For each tissue sample, 3 biological replicates were prepared; each biological replicate comprised of RNA extracted from tissues collected from 4 to 5 fishes.
Four biological replicates were prepared by pooling ten individuals in each replicate of either G or RB tissues, snap-frozen in liquid nitrogen, and stored at -80°C until RNA isolation.
Three independent biological replicates were prepared for sequencing using the TruSeq Sample Preparation kit (Illumina) and sequenced on the Genome Analyser II system (GAIIx, Illumina).
Six biological replicates were prepared for each tissue and sex, except the female brain, which consisted of 7 biological replicates and two technical replicates (but only 6 biological replicates were used for quantitative comparisons).
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