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All competitions were propagated for 7 days on solid agar with daily transfer of communities to fresh medium; in half of the replicates, population structure was maintained at each transfer, whereas in the other half of the replicates, the population structure was homogenized at each transfer.
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We consider a unicellular, asexually replicating population of organisms, whose genomes consist of a single, double-stranded DNA molecule, i.e. one chromosome.
Each replicate population comprised 1000 to 2000 mating individuals.
Each of the ten treatment protocols with an antibiotic was replicated eight times, while the control included 16 replicate populations, yielding a total of 96 replicate population per experiment (a total of 192 replicate populations for the two evolution experiments).
Each replicate population was used for only one population pair within a generation, except on two occasions, when a replicate population was used for two population pairs because of an odd number of replicate populations available.
Interpopulation crosses were carried out between randomly picked males from one replicate population and randomly picked females from another replicate population of the same size.
Replicate population identity was nested as a random factor within the treatment in the model.
This test was applied for each AR and TW replicate population.
After a total of 150 generations of growth, individual clones were isolated from each replicate population.
When mature, the parental flies for each replicate population were randomly picked among the respective offspring.
For all treatments we started each replicate population with 500 individuals.
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