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It transforms all the replicates onto the same scale.
Peptides were printed in six replicates onto streptavidin-coated slides (ArrayIt) using the VersArray Compact Microarrayer (BioRad).
For the cytotoxicity assays, 500 cells were plated in six replicates onto 6-well plates in complete growth medium.
The purified products were subsequently printed in eight replicates onto glass slides and quantified using Syto61 (Molecular Probes Inc, OR, USA).
Samples were injected in two 1-μL replicates onto a C-18 reverse-phase ultra pressure liquid chromatography (UPLC) column (ACQUITY UPLC BEH C18, 1.7 μm, 2.1 × 50 mm; Waters Corporation, Milford, MA).
For the unpaired analysis, the arrays within a given condition were linearly normalized to each other, in order to map intensities from different replicates onto similar scales without altering the absolute ratio values (as described above).
Similar(52)
Two hundred microliters of each suspension were loaded into individual wells within a Cathra replicator plate (Oxoid Inc., Ogdensburg, NY) and replicated onto a series of Mueller-Hinton agar plates amended with 2-fold increasing antibiotic concentrations.
Obstructing sightlines and walking paths, squeezing already congested sidewalks, the sheds have become pedestrians' analogue to squeegee guys, a minor obstacle replicated onto every street corner, at least when you're in a hurry.
Microstructures on the master mold were first replicated onto a PDMS negative mold using conventional soft lithography process.
Finally, the hierarchical structure of the etched Al plate was replicated onto the surface of a low density polyethylene (LDPE) plate by applying heat and pressure.
The silicon master pattern was then replicated onto a polytetrafluoroethylene (PTFE) polymer stamp by UV nanoimprinting to obtain the inverse pattern profile (i.e., an array of nanopillars).
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CEO of Professional Science Editing for Scientists @ prosciediting.com