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To confirm the lack of matrix effect on the VNT analysis, 6 various batches of both matrices were extracted and spiked with 20 ng/mL of VNT (LQC) and IS as set 1. Similarly, preparation of set 2 was performed, which consisted of 6 replicates of same concentrations of VNT and IS but solubilized in mobile phase.
Transgenic plants along with wild type plants having similar biomass (0.2 gm each) in triplicates (multiplied by micropropagation for getting replicates of same genotype) were transferred to Hoagland's medium [42] for 10 days for acclimatization.
The values obtained by this was background corrected and ratios of treated versus untreated intensity were converted in Log2 ratios which was averaged for replicates of same set of experiment.
Quantile-normalization is then applied within tissues on the assumption that probe intensities in different replicates of same tissue follow the same distribution.
Similar(56)
To assess whether this explanation is true or not, we normalized the microRNA microarray data within replicates of the same samples in the same platforms (Figure S2).
Multiple arrays hybridized using the same RNA or multiple replicates of the same gene within an array are referred to as technical replicates.
In particular, different lineages deriving from the same WGD act remarkably similarly as replicates of the same evolutionary "experiment" over hundreds of millions of years.
Samples were randomized by sex and age such that biological replicates of the same group were not hybridized to the same slide.
Differences in expression among the variants were on the same order as batch-to-batch variation among replicates of the same protein.
We found differences in the RSAP estimates between runs using replicates of the same landscape created in the program MOSAIC.
A low CV is desirable because it indicates a consistent peptide transition signal, in this case across the integrated peak areas of biological replicates of the same bacterial dilution.
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