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Moreover, it is relevant to note that the straightforward preparation of the biological samples necessary for MALDI MS profiling allows to perform with a modest effort a high number of replicates necessary for statistical analyses.
One of the reasons is the number of replicates necessary for accurate qPCR results.
Using this simulated effect size, we will then determine the number of replicates necessary to reach a power of at least 80%.
We will use this simulated effect size to determine the number of replicates necessary to reach a power of at least 80%.
We derived an empirical low gene expression threshold using both genic and intergenic read count data, and we estimated the numbers of biological replicates necessary to detect differential gene expression.
Determining the number of microarray replicates necessary for classification of expression profiles has been presented as an important issue [ 33, 34] and should be one of the first things to consider when designing any experiment.
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This variability affects the sharpness of the statistics, and a larger number of replicates was necessary.
How many independent EVC lines should be included to test for transformation effects, and how many replicates are necessary to detect significant ecological effects of single genes?
Data were averaged over years or replicates where necessary and a study that included more than one species, and was deemed independent, was included separately in the analysis (referred to as cases from here on).
Analysis of technical and biological replicates is necessary to ensure the accuracy and biological significance of proteomic datasets.
Multiple biological replicates are necessary to adequately assess the full extent of the variation in the biological system.
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