In this study, results obtained from one-step viral growth curves indicate that human r-RV replicate less than or equally to their normal counterpart.
Thus, human r-RV with rearranged segments 7R, 7RΔ and/or 11R replicated less than or equally to their wt-RV counterpart in MA-104 cell culture.
However, considering that r-RV (M1 to M4) replicated less than or equally to the wt-RV M0, though sharing a same genetic background, it was unlikely that r-RV could have overgrown wt-RV, especially when mixed-infections were performed at MOI ratios favoring wt-RV.
Previous studies also found that trans eQTLs replicated less frequently than cis [ 26, 27].
The R292K variant was observed to replicate less efficiently than the wild-type at day 3 post inoculation (2.32 versus 3.49 log10copy/μg RNA, P=0.05, Mann–Whitney U test).
Oseltamivir-resistant human influenza A viruses (H3N2 and H1N1) have been found to replicate less efficiently than oseltamivir-susceptible strains in cell culture and animal models (12 – 12 ).
Furthermore, evidence has showed that the viral titers of H5N1 and H1N1 were similar during the early infection, and then at later time points the H5N1 virus even replicated less efficiently than H1N1 did, ruling out the possibility that the higher mortality of the H5N1-infected cells is attributed to the more efficient replication [ 21].
Even if the 12 repeats of GGTTACA contribute to regulating plasmid replication efficiency or timing, they cannot be the only motif doing so, because plasmids lacking these repeats (∆C and ∆D, Figure 5, Figure 6) nevertheless replicate less efficiently and later than a plasmid containing only region R1.
BJ501 virus replicated less efficiently in the lungs than PR8, and both caused lethal illness in the mice.
