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In cultivation trials we used the maximum number of genotypes (instead of less genotypes in replicates) in order to generate as much genetic variation as possible.
In addition, we measured Luciferase activity for an empty (i.e., with no promoter) pGL4.14 vector, in five replicates, in order to estimate background Luciferase transcription levels.
For all PCR reactions, we used biological replicates rather than technical replicates in order to assess the robustness of the reactions using a variety of different templates.
Specifically, we first classify subject replicates at the sample-level and then perform a majority vote across the subject replicates in order to compute subject classification.
These small-scale site-specific differences reiterate the necessity to analyse numerous replicates in order to establish baseline bacterial community states for healthy coral populations.
Then one of the A/H1N1 and two of the A/H3N2 dilution tiers were expanded from three to twenty replicates, in order to demonstrate the high likelihood (≥95%) of positive detection and identification at the indicated dilution of the respective neat stocks.
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In the context of the EUDAT task force, only several data-sets are replicated in order to bring the 'date replication service' towards production meaning that some communities choose to replicate data from some of their specific scientific projects and not all of them.
Critical resources must be replicated in order to provide desired level of services.
In this situation the experiments should be replicated in order to improve the precision of the estimates.
Herein, the evolution of the pressure profile has been replicated in order to adequately extend this scheme for oil-well simulations.
Therefore, we evaluated whether earlier findings of lower striatal D2/3R availability in obesity can be replicated, in order to increase our understanding on the potential role of dopamine in obesity.
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