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The current study involved three independent biological replicates from each year's sample collection.
Three replicates from each nematode isolate were prepared for metal analysis.
All results were presented as the mean values of three replicates from each sampling time.
Biological replicates from independent cultures were assayed equally.
Data presented were from one experiment where three replicates from cultures at OD600nm = 1, 2 and 3 and two replicates from cultures at OD600nm = 4 were collected.
QC duplicates and replicates from each study were genotyped, blinded to laboratory personnel.
Threshold cycle number was determined for four biological replicates from each variant.
Each experimental condition included six biological replicates from the same overnight culture.
Replicates from different independent clones were prepared and hybridized to the microarrays on different days.
For each condition a minimum of 3 replicates from 3 different animals were run.
Each experimental condition consisted of six biological replicates from the same overnight culture.
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