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We reasonably assume that the use of experimental replicates does not change the basic conclusions of this work.
There were no technical replicates done.
Mean Ct dispersal for technical replicates did not exceed 0,3 cycle.
All immunoblot results were confirmed by at least three independent experimental replicates done in separate reaction tubes.
Data is expressed as percent of controls and is the average of three biological replicates done in duplicate.
Clustering of individual replicates did not produce any clusters.
The RR genotype in all replicates did poorly, indicating that having two R alleles is costly.
If both replicates don't give similar results, how robust are the conclusions?
Control and high-As3+ exposed high-As3+ exposedates exhibiologicalistent replicatesility among rexhibited, whereas low-As3+–exposed repliconsistentnot.
Using technical replicates does not reduce the throughput of the sequencing, and only marginally increases the cost of the experiment.
The clear supernatant was used for the measurement of enzyme activities with three replicates done for each assay.
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