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This suggested that stochastic variability among these technical replicates contributes little to overall sequencability (≥1x) although it had an appreciable affect on usable (≥20x coverage) sequence data.
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Moreover, the elevated number of eight replicates contributed to reliable results.
Reviewing the replicates contributing to each sample mean, we noticed a pattern that could be explained by vascular transmission of the JA burst: some replicates from distal sectors did not accumulate any detectable JA at all, while some replicates from the same sample group accumulated JA levels as high as those in replicates from SE*.
This dependency is expected to even out when the number of replicates is high, i.e. when additional replicates contribute very little to the variance estimation.
Although in each case the degree of improvement begins to plateau, even the addition of the final replicate contributes to reduction in error.
Because each of the four treatment groups comprised 12 independent RNA samples, these 12 were pooled into 3 major groups of 4 replicates with each replicate contributing 0.5 μg.
The generation of technical replicates additionally contributes to assay reliability.
We therefore concluded that inappropriate DSB formation on replicating chromosomes contributes to the loss of cell viability in checkpoint mutants.
Only fragments present in both biological replicates and contributed at least 1 % of the total fluorescence signal were included in the analysis.
Each of the replicate communities contributing to distributions and relationships in Figs 3 5 is represented by values averaged over time-steps 401 500, long after the asymptote of species richness.
Transcripts whose differential expression profiles are not shared between biological replicates may not contribute in the attenuation process.
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