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Also, ComBat and XPN give increased variability between replicates compared to standardisation in some cases, showing that cross-platform normalisation comes with a cost in terms of replicates.
This difference was statistically significant (P<0.01) for the plasmin-labeled replicates compared to unlabeled or plasminogen-only controls and suggests that C. neoformans may utilize plasmin, a potent serine protease, to facilitate its invasive potential across extracellular matrices.
We observe 85 Mbps in both replicates, compared to roughly 130 MBp.
The fold difference ± SEM (n is biological replicates) compared to wild-type RPE cells is indicated below.
The graph shows less dispersion between the filtered regions of the 12 technical replicates compared to the unfiltered regions.
The 24K panel revealed less variation between both technical and extract replicates compared to the 1.5K panel.
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The variance (standard error) was very small between the PCR replicates for a same biological sample (Real-Time experimental replicate) compared to the variance between the different biological replicates.
R and Kappa were slightly lower for the biological replicates as compared to the technical replicates and further decreased when comparing the two experimental conditions.
All experiments were performed with three biological replicates, and compared to three biological replicates of a J2315 control grown on Iso-sensitest medium without any antibiotics.
Comparisons of replicates were performed in order to account for variation of transcription values within a strain; importantly, the genes that were consistently differentially transcribed across replicates were found to be homogeneously transcribed when strain replicates were compared to each other.
In each case, the average radius of the expanding cell colonies, using three experimental replicates, was compared to the average radius of the expanding simulated cell colonies, again using three simulation replicates.
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