One gem was: "Always ensure that your practice replicates as closely as possible what happens in the middle".
However, SV40 DNA excised from the plasmid replicates as well as authentic viral DNA and is fully infectious.
Further, it is present in each set of replicates as well as the combined dataset.
So, once CHIKV 05.115 succeeds in crossing the midgut barrier, it replicates as efficiently as CHIKV 06.21.
Third, an objective coverage evaluation among technical replicates as well as different samples was performed.
Measurements comprised four biological replicates as well as four technical replicates.
All assays were performed in three technical replicates, as well three biological replicates.
Similar approximately linear relationships are observed for the other replicates as well.
The number of replicates as well as the number of independent experiments are indicated in the figure legends.
They find that NuGEN performs remarkably well across multiple laboratories and across technical replicates as low as 500 pg [ 28].
We consider simulated and laboratory-generated replicates as well as resampling replicates from a real crime case.
