Exact(5)
All data represents mean ± SE of three replicates and differences between means analyzed using DMRT test (P ≤ 0.05) All data represents mean ± SE of three replicates and differences between means analyzed using DMRT test (P ≤ 0.05).
All data represents mean ± SE of three replicates and differences between means analyzed using DMRT test (P ≤ 0.05).
Notice that these are not true replicates, and differences in response among selection lines can be due to differences in their genetic features, as well as in differences in the stochastic events associated with mutation, drift and selection.
A heat map analysis of miRNA-Seq data comparing the eight samples showed uniform expression pattern within the biological replicates and differences between MDCK and MDCK-Ras cells (Additional file 9: Figure S3B).
The variability between types of mass spectrometers, differences in LC-gradient between technical replicates, and differences in individual glycopeptide preparation methods might have resulted in imperfect reproducibility between technical and biological replicates.
Similar(55)
Although the subgroup hypothesis was made a priori and differences are in the anticipated direction, the analysis is based on between group differences, has not been replicated and differences between results with the two devices is easily explained by chance (p=0.22).
Means of six replicates and different letters indicate statistical difference (Duncan's test at p ≤ 0.05).
The reproducibility of the assay was demonstrated across multiple biological replicates, and notable differences in phosphoprotein expression were observed between different cell lines.
The expression level of each gene was represented by the mean of the log2-transformed, normalized and median polished signal levels across each set of tissue replicates, and significant differences between mutant and wild type were determined using linear models in the Bioconductor LIMMA package with a Bayesian correction for standard error.
Principal component analysis plots for leaf and cotyledon samples show consistency between biological replicates and significant differences between the stages selected for comparative analysis (Additional file 3).
Principal component analyses based on these DEGs confirmed the correlation between replicates and the differences between cells at the three stages of reprogramming.
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