Exact(2)
First, proteins had to be detected reproducibly in at least one of the 4 types of samples analyzed, i.e., present in at least 2 of the 3 replicates acquired per sample type.
In order to quantify accurately and consistently the diffusion constant of each protein species, at least eight separate replicates acquired on two or more days of experiments were analyzed for each protein species.
Similar(58)
For each peptide group, three transitions were monitored; and for each transition, four replicates were acquired.
A total of 3 technical replicates were acquired for each of the 4 types of samples analyzed (early trophozoite with and without bafilomycin A1 treatment and late schizont with or without bafilomycin A1 treatment).
Is it exclusively yours or can it be easily replicated or acquired by a competitor?
PP conceived, designed, and performed replicate experiments; acquired, analyzed and interpreted the data; wrote and revised the manuscript.
Bootstrap values from 1000 replicates were also acquired.
Data for qRT-PCR are presented as mean ± standard error of the mean of a minimum of 2 and maximum of 3 replicates of measurements acquired from 4 donor specimens (N = 4, n = 10).
High throughput LC-MS analyses are then performed for a large number of biological replicates and the acquired datasets are compared with the database to identify the peptides that are actually present.
During this unique biphasic developmental cycle replicating bacteria acquire energy and biosynthetic precursors from the infected cell.
Identical conditions were used for all proteins tested, and in most cases replicate data sets acquired (Table S1).
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