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From these master plates, a sample was replicated with a 96-well replicator to a microtitre plate containing YEPD and grown for 3 d.
A second batch was pregrown on YEPD to exponential phase, replicated with a 96-pin replicator to fresh prewarmed YEPD, and incubated for 6 hr at 30° before assay.
After 3 d, colonies from the agar plate were replicated with a 96-well replicator to a prewarmed microtitre plate containing YEPD, incubated for 6 hr at 28°, and used for the heat-shock assay of cells in exponential phase.
Cultures were replicated with a 96-pin metal replicator (Sigma-Aldrich) on 4 types of solid plates: complex media with or without H2O2 (5 mM), defined media, or glycerol-containing media (defined media with 1% glycerol, 0.1% glucose).
Better yet, it's a look that can be replicated with a single product and is basically impossible to mess up.
"They are cheaper... [but] their designs are stolen and replicated with a lower quality and it is killing their businesses".
They rarely arrive in a baby-manual-ready bundle, already replicated, with a massive data set backing up the conclusions.
But putting Mrs Clinton on the ticket would produce few benefits that could not be replicated with a carefully chosen alternative vice-president.
Let's hope that the results at San Jose State will be replicated with a scaling up in class size, and let's be sure we take away the right lesson.
These experiments should be replicated with a bigger sample size.
However, many of the features built into this watch can be replicated with a good app.
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