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Replication of the rcAd26 vectors was species specific, as these vectors did not replicate in the rhesus monkey cell line MK2 (Fig. 1C), whereas a replication-competent simian Ad vector derived from rhesus monkeys (21) replicated well in MK-2 cells.
As shown in Figure 1(b), VSV-GFP replicated well in cell culture supernatant from mock-infected cells, while VSV replication was effectively inhibited in those MDBK cells that were preincubated with supernatant from SeV-infected EECs.
All four deglycosylated mutants replicated well in rhesus peripheral blood mononuclear cells (PBMC) in vitro, and the replication kinetics were similar to SIVmac239 ([2], and data not shown).
The magnitude of the determination coefficient shows that observed outcomes are replicated well by the model.
Conversely, L135W replicated well in C6/36 cells, but had the lowest mosquito infection rate.
Breakthrough curves (BTCs) of colloids and SAs were replicated well by a newly developed numerical model that considers colloid-filtration theory, competitive kinetic sorption, and co-transport processes.
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The average value from the three replicated wells was used as data for each biological replication.
Each determination of proliferation was carried out in at least five replicated wells.
The obtained absorbance values were further corrected for cell numbers measured in replicated wells.
The concentrations were corrected for cell numbers measured in replicated wells in parallel.
The attenuated Gt strain, however, is not virulent in chickens and replicates well in CEF cultures.
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