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Because we lacked replicated values of connectivity for given values of natural vegetation, a formal test of lack-of-fit of a simple linear model (Kutner et al. 2004) was not possible.
Replicated values of each gene were then averaged.
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For each gene, standardized quantity values were calculated by dividing the mean of the quantity values for the three technical replicates for each sample by the mean of the replicate values of the RCP1 exogenous control for that sample.
SMILE mRNA expression was checked by RT-PCR and decreased by almost 84% in resting HeLa cells transfected with SMILE siRNA as compared to cells transfected with the Stealth RNAi negative control Low GC (Figure S6, ***p = 0.0002, Mann-Whitney test, mean replicate values of three independent experiments).
SMILE mRNA expression was checked by RT-PCR and decreased by almost 70% in resting keratinocytes transfected with SMILE siRNA as compared to cells transfected with Stealth RNAi negative control Low GC (*p = 0.0418, Wilcoxon signed rank test, mean replicate values of four independent experiments, data not shown).
For every study subject six replicate values of relative expression per each alternative WNK1 splice form (ex+11+12, ex−11+12, and ex−11−12) was calculated.
Three replicate values of the cell death phenotype were collected for 173 HapMap cell lines in both uninfected and Salmonella infected samples.
The poor correlation of replicate values of the three male lung- versus-spleen arrays (Pearson R2 of 0.22 to 0.59) is likely because almost all of these values are close to zero, and variation is therefore predominantly experimental noise.
We thus found 1,199 genes with statistically significant differences among the replicate values of fold-changes of the two separate patient subgroups (P < 0.05; false discovery rate 5%) (Additional file 1: Table S1).
The irony inherent in the old stereotype is that those fleet-fingered, impersonal performers (yes, there were some) replicated the values of a Western conservatory system that has long emphasized sheer technique at the expense of all else.
Each combination of salinity and temperature was conducted in two vessels per run, with an average of the vessels used to create a replicate value of each treatment regime.
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CEO of Professional Science Editing for Scientists @ prosciediting.com