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This model derived from the BWHHS data was replicated using data from the "usual care" arm of a large randomised trial of health care in general practice for people aged 75 and over.
Alternate distance metrics and clustering methods were also tested, and these tests can be replicated using data from Additional file 9. Our tests with other methods did not reveal major differences, except that Ward's method of clustering produced very homogeneously-sized groups, as it is known to do [ 42].
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This result was replicated subsequently using data from the Midlife Development in the United States Survey (MIDUS), which employed a brief predictive version of the CIDI and also included self-reported diagnoses of arthritis [ 8].
We could not replicate rs4961149 using data from three of the non-CGEMS cohorts.
We sought to replicate associations using data from the Eldermet cohort [ 15, 16], a study that used different methods for sequencing and taxonomic assignment and quantified frailty differently using the Barthel index.
Future research should seek to replicate this approach using data from other universities.
This investigation has attempted to replicate these novel findings using data from the Avon Longitudinal Study of Parents and Children (ALSPAC) cohort.
A marker was 'replicated' when it was identified to be statistically significant in Study 1 followed by replication in Study 2. For replicated markers, a combined analysis using data from both studies was carried out to evaluate the overall effect.
Results from Look AHEAD generally agree with our findings, and selected findings in the current study were replicated in a previous study using data from NHANES 1999 2006 (20).
Tai et al. ranked DE genes using data from replicated microarray time course experiments, where there are multiple biological conditions [ 15].
To validate the findings, we replicated the analysis in mouse using data from the Mouse Genome Database (MGD) [ 17] and obtained similar results (Table 1, Additional file 1: Figures S2). a 95% confidence intervals are in brackets.
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