The lysogenic lifestyle results in the establishment of the phage genome inside the bacterial host where it can remain in a dormant state replicating together with the bacterial chromosome.
Through cascading expression of different gene groups the virus genome finally replicates together with other virus components into cell vesicles.
The capacity of CACs to replicate together with their ability to undergo ductal and endocrine differentiation indicate that they can behave as adult pancreatic progenitor/stem cells in vivo.
At each temperature and time point, triplicate biological replicates together with a blank control were prepared.
Recombination was investigated by split decomposition analysis using SplitsTree version 4.8 [ 44] with default settings (uncorrected P method) and 1000 bootstrap replicates together with Phi test for recombination [ 45].
Hereafter, the sensitivity and specificity was evaluated by analyzing ten wild-type replicates together with a standard dilution series of mutant alleles into wild-type alleles (50%, 10%, 1%, and 0.5%) in triplicates.
In order to obtain robust, valid comparative RNA expression values, total RNA was extracted from the same batch of cell lines, converted into Cy5-labeled cDNA, and hybridized in replicates together with a common Cy3-labeled reference, to both cDNA (30 k) and oligo (34 k) spotted microarrays.
Viability of the cells under each condition was determined as mean of 3 to 8 replicates together with its standard deviation and then standardized on the result of the double control (no cytotoxic agent and no DEX applied); i.e. the viabilities of all conditions were divided by the mean of the double control.
The average (AVG) expression ratio of the mutant sample to the wild-type control was calculated from 12 replicates together with the respective standard error (SE), a standard t-test result (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; *****, P < 0.00001 probability in Student's t-test).
Table 2 presents the descriptive statistics for these replicated values together with the newly created HRL indices, average student mathematics achievement scores, student sample sizes, and school sample sizes.
