Further evidence that the presence of CpG dinucleotides in viral sequences either activate or are targets of cell defence mechanisms is provided by the observation that polioviruses with artificially elevated CpG frequencies in their genomic RNA were markedly attenuated and replicated to titres several orders of magnitude lower than wild type virus in in vitro cell culture [ 34- 36].
Our model and statistical method will need to be replicated at several institutions to test its ability to describe workload and learning in other settings.
Experimental infections of fruit and insectivorous bats showed that filoviruses replicate to high titres in several organs (Swanepoel et al, 1996).
Hadoop manages its own file system, the Hadoop distributed file system (HDFS), which replicates data to several nodes to ensure the availability of data.
This intermediate virus can be isolated by allowing the virus to replicate during several passages and to be amplified in the presence of the antibiotic, which causes cells to detach and does not allow plaque assays.
However, to qualify them as longevity-regions, these associations still need to be replicated in several larger independent cross-sectional and prospective studies.
We demonstrate proof-of-principle by using the gene-based test to replicate several genes known to be associated on the basis of results from a family-based GWAS for height in 11,536 individuals and a DNA-pooling-based GWAS for melanoma in ∼1300 cases and controls.
First, such an approach requires the existence of genetic variants that have been shown to be robustly (replicated in several independent studies) associated with the non-genetic modifiable exposure of interest.
This new energy budget approach, which was replicated by several subsequent studies, seemed to indicate the Earth's climate is a bit less sensitive to carbon pollution than previously thought.
To rule out the possibility that individual differences in sensitivity to caffeine between mouse mothers or pups didn't lead to any misleading effects, the work needs to be replicated several times in much larger groups, and in different breeds of mice, Silva says.
