The DNA viral metagenomes from 22 environmental samples were obtained from their respective databases as listed in Rosario and Breitbart (2011) and each metagenome was de-replicated to remove duplicate sequences.
Post-PCR analysis was performed to mark failed samples with a Ct value greater than 40 in over 50%% of replicates and to remove unreliable samples with coefficient of variance greater than 30%% in Ct values across replicates.
As for shutdown decisions, he said, unlike parents, officials need to be "dispassionate" about them, because the city believes the best way to improve public education is to identify the high-performing schools and try to replicate them, and to remove students from consistently low-performing schools as fast as possible.
3. Physiological condition On Days 0 and 120, L. elliptica from each experimental replicate were dissected to remove flesh, the flesh wet weight determined, and the flesh and shell oven dried (60°C) and air dried, respectively, to constant weight (hereafter 'FW' and 'SW', respectively).
However, in a replicated server-based system there are possibly more than two replicated servers and we want to remove a machine as soon as possible.
Can the war-footing kind of effort to remove polio be replicated?
Minimal number of replicates serves as a threshold to remove genes without sufficient number of observations.
To infer the final tree shown in Figure 1, we first used the GUIDANCE server [ 75] with 100 replicates of PRANK and removed columns with less than 5% support, in order to remove alignment columns that were likely to have been misaligned while retaining most of the potentially phylogenetically informative columns.
Things are becoming easier and easier to codify and replicate, while removing the viewer from the process.
The virus inserts itself into the genome of the host cell in order to replicate and then removes itself to infect other cells, sometimes capturing a portion of the host cell's genome along with its own.
Concurrent to removing test pegs for the indirect qualitative outgrowth test, replicate sample pegs were removed and directly examined for viability by the quantitative viability counting method and direct qualitative outgrowth test for comparison.
