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The 48 variable combinations were replicated to provide a total of 96 survivor curves from the experimental data.
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A plaster model of a pretreatment oral cavity was replicated to provide 18 (9 upper and 9 lower) identical versions.
That study revealed extensive changes in translation during parasite development, but lacked the biological replicates to provide a robust statistical analysis of the stage-regulated changes.
Analyzing transcriptional profiles with RNA-seq allows us to evaluate "snapshots" in time of bacterial transcriptomes; therefore, it is essential to generate data from more than one replicate to provide a broader more reliable picture of transcriptional changes.
The 38 variable combinations were replicated twice to provide a total of 76 survivor curves, which were modelled by a modified three-parameter Weibull function as primary model.
Replicates were averaged to provide a single value for analysis.
The average between the true mutation proportions of each technical replicate was used to provide a rough estimation of the total number of each type of mutation.
Thus, once an initial estimate of target quantity has been determined using real-time qPCR, a sample is diluted near to a predicted 0.5 N per aliquot and 16 48 replicate amplification reactions conducted (the more replicates, the greater the resolution [10]; however, as few as 16 replicates have been found to provide a reasonably reliable estimate).
First, permutation requires a large number of replicate samples in order to provide a reliable P-value estimate, whereas we wish to analyse experiments with small numbers of replicates.
And several nonvirulent strains appear to replicate well enough in chimps to provide a realistic challenge, they wrote.
This systematic review replicates an earlier study, aiming to provide a comprehensive update of the most current developments in interventions for children affected by armed conflict.
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