That was not the aim of this study, and we preferred to pool the model and landscape replicates to have sufficient degrees of freedom for the statistical analyses.
Using quantile normalization, we transform microarray estimates, taking the median across sample replicates, to have the same distribution as the sequencing estimates.
A necessary (intrinsic) condition for genotypes to be replicated is to have a 3′-dangling end on both strands.
For example, the AUX Viewer program has been replicated to run in the MATLAB environment using AUX MEX.
Our approach requires careful experimental design with many replicate cultures to have sufficient data points over important phases of a growth curve.
We calculated the minimum number of replicates needed to have a robust loess procedure.
Each probe was printed in triplicate to ensure the presence of internal replicates and to have a good statistical representation of each transcript on the array.
"That is the most important step -- to replicate this and to have it in a much larger sample". .
How Ferguson senior would like to have replicated his son's run of results this month.
In early cells RNA is thought to have replicated itself in this way.
