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Stages exhibiting a greater need for computation are replicated to balance the time spent on each pipeline stage.
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Ten replicates are chosen to balance runtime and accuracy.
Pooling of biological replicates does not allow statistical comparisons, but produces sufficient sample to enhance the detection of low-abundance proteins through technical replicates and helps to balance the between subject variability.
This time, however, for the derivation of the unified datasets we replicated the image features 10 times, so adding 310 replicated image features, in order to balance the feature size effect with the microarray features.
Collectively, the above results indicate that in order to achieve optimal levels of silencing from a replicating HSV, it is important to balance the levels of shRNA/miRNA expression against cytotoxicity caused by the virus.
We generated eight technical replicates with the Ribo-Zero-Seq-FFPE protocol to balance the total number of transcriptome reads for the comparison with FF mRNA-Seq.
To balance the duration of different stages, the longer stages have to be replicated.
In order to balance the data, they used the Smote algorithm [ 13] to replicate minority class samples.
Therefore, we used the mean values of the two replicates to achieve balanced data in all analyses (i.e. when missing values were present mean values were replaced with the value of the other replicate).
Just to balance the hurt.
How to balance all this?
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com