Forward mapping is performed to replicate the expression profile of BA-BpCAT for verification of endo16 SLM.
Notably, the use of a sense riboprobe corresponding to hybrid grn does not replicate the expression pattern observed for ASgrn1-2.
This module was validated in an independent material, by replicating the expression changes in allergen-challenged CD4+ cells.
To replicate the expression in the hermaphrodite and determine expression in the male, we fused the unc-18 upstream sequences used in the mutant-rescue experiment to a YFP reporter.
In addition, dsp-4 pretreatment increased inducible nitric oxide synthase expression solely in neurons rather than in microglial cells, more accurately replicating the expression pattern seen in AD patients [ 41].
Exposure to hydrogen peroxide replicated the cell expression of the senescence marker p21WAF1/Cip1 observed in vivo.
We replicated the reported expression differences across the C57BL/6 J vs. DBA/2 J strains for the five miRNAs we previously found to be differentially expressed [ 20].
Moreover, biochip analysis replicated the higher expression of both quantitative markers Tm1 and MaoB (Fig. 2c) in patients as compared to controls.
We observed a differential expression of the four different UCPs in control fibroblasts and SHSY5Y cells which replicated the tissue-specific expression of these genes previously reported (48, 49).
However, previous reporter analyses showed the POS9 element to be sufficient to replicate the INO expression pattern (but at a lower level of expression) when present in four or more tandem copies [ 12].
If the trends observed in this comparison with prior data were applicable to C. elegans genes more generally, beyond our selected set of transcription factor genes, then 90% of Promoterome inserts will drive expression in endogenously significant domains, with almost half fully replicating the complete expression pattern.
