Three out of the four Asian cohorts of IGGC did not replicate the direction of effect for rs2286885 (Supplementary Material, Table S2 ), a finding that could reflect differences in LD pattern at this locus between European and Asian populations, justifying our decision to include only the European cohorts in the replication.
"I wanted to experiment with lighting the cannonballs from various directions, replicating the directions of the sun and time of day.
Associations were considered replicated if the direction in the replication analysis was the same as in the discovery analysis and the one-sided P-value was <0.002, corresponding to 0.05 divided by the number of SNPs tested, n = 25 (Bonferroni correction).
Further, for replicated hits, the direction of the association with methylation was highly concordant (98.5 100%%) with previous studies.
The Australian Twin Eye Study and BMES both replicated the 5q21.2 CNV association and direction of effect (P = 0.001 and P = 0.02, respectively).
For the gaze-center condition, we replicated the topography related to saccade direction that has been previously demonstrated in the human PPC (Sereno et al. 2001; Schluppeck et al. 2005; Konen and Kastner 2008), although our maps did not exhibit the very gradual progression of phase values of these previous studies.
Finally, the oriented cells formed linear groups that were aligned parallel to the direction of the strain and replicated the native skeletal muscle cell patterning.
Protein-abundance changes were strengthened when the protein was identified in both biological replicates and the direction of change in both replicates agreed.
They're heading in precisely the opposite direction, replicating the failing work programme, outsourcing service delivery to a handful of large private providers while local probation trusts are abolished and long-established working relationships ripped apart.
qPCR analysis (pooling samples and independent RNA extractions from biological replicates) confirmed the direction of change detected by DGE analysis.
