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To minimize the effect of biological variation between ears on gene expression, equal numbers of dissected endosperms from 4 ears were pooled and treated as one sample; thus a minimum of three replicated samples was used for each experiment.
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In this study, five replicated samples were used from each treated and untreated sample.
For 'Holiday', two replicated samples are presented.
The mean of all replicated samples is meaningless in this context, and is not shown.
For each setting of (p, β ij, N), 10 replicated samples were generated.
Addition of 3,35,5-tetramethylbenzidine 3,35,5-tetramethylbenzidinellowed by measurementoof absoreplicate 450 nm.
The loci included in replicated sampling were selected randomly using Excel's RAND function.
From 186 individuals replicate samples were performed (between two and five replicates per individual).
The measurement error of the equipment for replicate samples was less than 1%.
Analytical precision for standards and replicate samples was ±0.2‰ (1σ) for oxygen and ±0.3‰ (1σ) for sulfur isotopes.
Routine precision for replicate samples was ± 0.1 ‰ for δ15N and ± 0.3 ‰ for δ13C.
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