Differences among sites in sampling effort (number of sampled years and replicated samples within years) were significant and accounted for 9% and 7% of variation in genus and family data, respectively (RDA).
The replicated samples produced only one discordant genotype among 107,412 genotypes indicating an assay replication success exceeding 99.99%.
CIs were computed as bootstrap 95% percentile intervals based on 10000 replicated samples.
In this study, five replicated samples were used from each treated and untreated sample.
To obtain the reproductibility of RT-PCR, the experiment was repeated 2 times with 2 independent biological replicated samples.
Six replicated samples at each level were prepared and analysed under the same experimental conditions during the same day and on different days.
We examined the muscles from replicated samples of Cirrhinus mrigala as a bio-indicator of water pollution following sewage and industrial discharges from the Lahore city into Ravi River of Pakistan.
Sampling followed a Before-After, Control-Impact design, with replicated samples being collected at one control site and two treatment sites, both before harvesting and then again seven to eight years after the harvest that established the WHS.
It consists of 24 mussel genes involving both normalizing elements and stress response related genes, each represented on the array with one or two different 50 mer oligonucleotide-probe reporters spotted in replicated samples on glass-activated slides.
The calculated error rate was approximately 3% based on negative controls and replicated samples.
This exceeds previously published metatranscriptomic studies because of the inclusion of replicated samples.
