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Firstly, the accumulation of fully replicated products is relatively slow, occurring over 30 45 min, yet significant accumulation of replication intermediates does not occur during the time course.
A time-course experiment was conducted to examine the kinetics of DNA synthesis in this system, and replicated products were analysed by electrophoresis in a native agarose gel (Fig 4A).
Thus, the replicated products were predominantly the same length as the full-length template.
Locations of the control DNA were determined by ethidium bromide staining, whereas the positions of the radioactive replicated products were detected by autoradiography.
At later time points, a slight decrease in product amount was observed (Fig 4A, lanes 6 8) perhaps due to degradation of the replicated products.
Therefore, we next performed a titration of the non-specific competitor, poly dA-dT), which was used because it will not interfere with detection of poly dA-dTorporation into replicated products.
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Companies that once sold easy-to-replicate products to nearby car companies are being bypassed by distant rivals in China.
Five replicate products from each starting quantity were precipitated with either 2.5 M LiCl, column, or guanidinium methods (figure 4D).
If a DNA molecule with such gaps is replicated, the product would be two sister chromatids with DSBs located ≤500 bp apart.
The role of a 21st-century photography museum should be to analyze, synthesize, and historicize our understanding of today's dispensation of images, rather than just to replicate the products of the search engine and the hashtag.
For example, a sufficiently large and ongoing decrease, made possible through technological advances, in what Marx called "socially necessary labor time," that is, the amount of time required for the production of a single type of product, if replicated across enough different products, could by itself bring about a lengthy postponement of the system's collapse.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com