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Two hundred microliters of each suspension were loaded into individual wells within a Cathra replicator plate (Oxoid Inc., Ogdensburg, NY) and replicated onto a series of Mueller-Hinton agar plates amended with 2-fold increasing antibiotic concentrations.
Obstructing sightlines and walking paths, squeezing already congested sidewalks, the sheds have become pedestrians' analogue to squeegee guys, a minor obstacle replicated onto every street corner, at least when you're in a hurry.
Microstructures on the master mold were first replicated onto a PDMS negative mold using conventional soft lithography process.
Finally, the hierarchical structure of the etched Al plate was replicated onto the surface of a low density polyethylene (LDPE) plate by applying heat and pressure.
The silicon master pattern was then replicated onto a polytetrafluoroethylene (PTFE) polymer stamp by UV nanoimprinting to obtain the inverse pattern profile (i.e., an array of nanopillars).
The employed metallic gratings are fabricated by interferential lithography and replicated onto resin substrates by soft-lithography techniques, thus thermally evaporated.
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By Pulsed-NIL we were able to replicate onto the hybrid perovskite structures with details at the sub-100 nm scale, in spite of its crystalline nature.
To obtain the rcn1 rcn2 double mutant, we disrupted the RCN1 gene in the rcn2 mutant background (YC581, the SAT1 flipper was removed via culturing YC531 in YPD medium and replicating onto nourseothricin-containing medium to confirm the loss of SAT1 flipper).
The resulting spores were plated on SFM agar without antibiotic selection and incubated at 30°C, and the resulting colonies were replicated consecutively onto SFM agar plates containing just kanamycin, just apramycin, or neither antibiotic, which were then incubated at 37°C.
After the passage of the replication fork, canonical nucleosomes are randomly distributed onto replicated daughter strands, leaving gaps that are filled with newly synthesized histone proteins (H3, H4, H2A, and H2B) (Smith and Stillman 1991).
In the nucleus, ASF1 "hands" the H3-H4 hetorodimer thethe CAF-1 compriorprior to the deposition onto replicated DNA, in the presence or absence of MCM2.
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