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However, it is a potentially important finding which, if replicated, needs to be understood biologically.
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Most parameters could be replicated without need for change.
Decreased susceptibility to Gram-negative bacteria observed in the GG genotype patients in the derivation cohort did not replicate; this needs further replication tests in larger cohorts.
Based on this typical variability and a components-of-variance approach, the relative number of replicates needed to provide experiments of equivalent power and sensitivity was determined.
And field replicates need to be analyzed by P-NMR as much as possible, rather than compositing samples, to allow statistical analyses of P-NMR results along with other field data.
Finally, these results were used to determine the minimum number of isolates and replicates needed to obtain a reasonable estimation, i.e. inside the confidence interval, with a probability of 0.8, 0.9 and 0.95.
Future manipulations should also be done on a longer time scale, and the replicates need to be spread over larger areas to capture the natural variations within the ecosystems.
We then describe a practical approach, useful to both stove testers and designers, to assess the number of replicates needed to obtain useful data from previously untested stoves with unknown variability.
To determine the number of replicates needed for each eat mutant strain in the feeding assay, the pilot study data were used in a mixed effects model [32].
At higher heart rates still, the number of replicates needed continues to fall (Fig. 8).
We calculated the minimum number of replicates needed to have a robust loess procedure.
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