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For both inoculation routes the highest viral loads were observed in lymphoid organs, indicating a progressive infection and replication of the virus in these organs, whereas calpox virus replicated less efficiently in organs of the digestion tract and the brain.
Several RNAi lines displaying strong proliferative defects also replicated less well in the uninduced state (Figure 3), probably from incomplete repression of the RNAi construct.
Thus, human r-RV with rearranged segments 7R, 7RΔ and/or 11R replicated less than or equally to their wt-RV counterpart in MA-104 cell culture.
However, considering that r-RV (M1 to M4) replicated less than or equally to the wt-RV M0, though sharing a same genetic background, it was unlikely that r-RV could have overgrown wt-RV, especially when mixed-infections were performed at MOI ratios favoring wt-RV.
Previous studies also found that trans eQTLs replicated less frequently than cis [ 26, 27].
BJ501 virus replicated less efficiently in the lungs than PR8, and both caused lethal illness in the mice.
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In this study, results obtained from one-step viral growth curves indicate that human r-RV replicate less than or equally to their normal counterpart.
However, further studies involving larger numbers of chickens and additional heterologous challenge viruses will be necessary to determine which of the two vaccine viruses is superior: namely, the NDV-HA virus that replicates somewhat better but incorporates a lesser amount of HPAIV HA, or the NDV-HAF virus that replicates less well (and is more attenuated) but incorporates a greater amount of HA.
In vivo the virus replicates less efficiently in the lung and, whereas the early stages (day 5) in the establishment of a latent infection are similar to wild type virus, by day 14 the number of latently infected cells as determined by the infective centre assay is greatly reduced and the viral DNA loads are around 10 fold less than wild type.
Therefore, M2C PrPSc may replicate less efficiently and take a longer time to propagate compared with the other PrPSc strains.
The R292K variant was observed to replicate less efficiently than the wild-type at day 3 post inoculation (2.32 versus 3.49 log10copy/μg RNA, P=0.05, Mann–Whitney U test).
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