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We performed three independent experiments with three different microfluidic chips replicated from the same master.
In the context of RNA sequencing, multiple nested levels of technical replication exist depending upon whether it is the sequence data generation, library preparation or RNA extraction technical processes that are being replicated from the same biological sample.
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Biological replicates from the same developmental stages were averaged.
Technical replicates from the same library are typically not necessary, due to the high technical reproducibility achieved by the sequencing technology [ 17].
As expected, sample A (100% UHRR) replicates from the same platform were found to be more highly correlated with sample C (75% UHRR, 25% HBRR) replicates than the other samples.
We then examined all replicated representations from the same source as annotated to reduce down to 57 ovarian cancer cell lines or 205 patient samples (from each source) by taking the median protein expression level of all replicates.
After this step we derived two consensus haplotypes for each monkey, splitting replicated haplotypes from the same monkey into two clusters, corresponding to two chromosomes, based on the Hamming distance between haplotypes.
Of particular notice, samples having initial higher fluorescence in baseline cycle, as shown in replicate D3 and replicate D5 from the same sample in figure 1a, are inclined to have higher fluorescence reading in the ensuing cycles.
To further evaluate our models we present confidence intervals obtained from bootstrapping for each analysis to account for potential pseudo-replication introduced by using temporal replicates from the same pond.
The variations between replicates from the same site were smaller than the variations between samples from different localities or between different mollusk species.
It is noteworthy that replicates from the same feeding treatment were projected relatively close to each other, thereby revealing a good reproducibility of the proposed method.
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