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The 288 deletion is flanked by short poly-A sequences, which might be responsible for this replicated error.
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For the SNP genotyping, we performed data cleaning, filtering, SNP calling, and replicate error analysis with GenomeStudio.
I first read it in Geoffrey Grigson's Faber Book of Love Poems, where line 12 was inadvertently omitted, and I've cheerfully replicated the error several times since.
The results shown are averages from three biological replicates; error barss indicate the standard deviation.
Statistical analysis shows that the difference between slopes is significant within the dataset but that the deviation is within that assessed previously for replicate error using DLS to describe particle systems changing in time.
The OOB replicate error rate estimate is always computed.
GeneSpring's replicate error model was used for our analysis.
Results are the mean of three biological replicates; error bars show standard errors of the mean.
For the SNPs genotyped, all genotypes between duplicates were consistent (0% replicate error rate).
Values represent the means of three replicates; error bars show standard deviation.
(C ) Quantitation of RNase H cleavage (average of three replicates, error bars represent standard deviation).
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