The strategy of congenic breeding was used to replicate detection of the chromosome 4 QTL and to assess its contribution to the infection phenotype.
These findings are controversial, because other laboratories have been unable to replicate detection of MMTV and because the DNA amplified by PCR in some laboratories has had homology with nonviral sequences in the human genome (summarised in Mant et al (2004)).
Replicated detection/non-detection occupancy surveys were used to estimate site use by leopards in a representative area of the park, and to investigate relative impacts of environmental, conspecific and anthropogenic factors on leopard occurrence.
GPNN was able to replicate the detection of a gene-environment interaction that had previously been detected using an exhaustive method, Multifactor Dimensionality Reduction (MDR) [ 59].
In parallel, we have developed a novel strategy to analyse the data that uses both genotype and pedigree information of all AIL individuals to replicate the detection of and fine-map QTL affecting juvenile body weight.
Replicating the detection of the QTL and fine-mapping their location with an AIL strengthen the original findings, and validate AIL as a valuable tool to explore the genetic basis of complex traits.
In order to maximize the power to replicate QTL detection and fine-mapping using an AIL produced from outbred founders, we propose that the genotypes and phenotypes of all the individuals in the pedigree and not just of those from later generations should be collected and analysed.
The relative standard deviation for 11 replicate detections of 15 μM histidine was 2.7%.
Analyses of 36 replicate samples yielded 85 paired-replicate detections of analytes, and 6 unpaired replicate detections (a detection in only one of the paired samples).
We, and others, have previously demonstrated that methylation of CpG loci detected through BeadArray platforms can be reliably replicated using alternative detection techniques including pyrosequencing, mass array analysis and quantitative methylation-specific PCR [ 50- 56].
Here, we performed simulations to build a simple statistical table of general guidelines adapted specifically for identifying differentially expressed genes using replications of a microarray experiment, and we present an accompanying nonparametric method, replicated studentized-deviate detection (RSD).
