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It has been suggested that the discontinuous replication of the lagging strand by the Okazaki fragments increases the frequency in which the template of the lagging strand is in single-strand (ss) DNA conformation relative to the template of the leading strand, which is replicated continuously (Frank and Lobry 1999).
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A significant challenge with the use of RT inhibitors is the development of resistance; because HIV replicates continuously at a very high rate, there are many chances for mutation and hence the emergence of a virus resistant to many drugs.
Cells from that biopsy became the first to successfully replicate continuously in culture (and hence be called immortal), and have since played a crucial role in many scientific breakthroughs.
Even during this period of clinical latency, the virus replicates continuously and causes new rounds of infection.
One explanation consists in the different way of replication for the two strands the leading strand replicating continuously, and the lagging strand replicating through Okazaki fragments.
The project, funded by the Wellcome Trust, has used more than a hundred spare IVF embryos from fertility clinics to establish several embryonic stem cell lines that replicate continuously in the laboratory.
In the above-mentioned field-based experiment, replicate plots continuously received either ambient or simulated increased atmospheric nitrogen deposition over a 14-yr period beginning in 1994.
The startup estimates that about half of all the code in the world is used for internal company procedures, yet most of the process is continuously replicated because the code is so similar.
Mutations anywhere in the mtDNA might have an adverse effect because the entire mtDNA genome is expressed and continuously replicated.
This high level of activity and relatively complex landscape of genome organization is remarkable given that an hour earlier the genome was being continuously replicated and doing little else.
Repeat continuously.
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