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The experimental designs followed augmented designs including trials and blocks within replicated checks, but unreplicated entries.
Quality control preprocessing of SNPs was performed by eliminating SNPs that did not match the following criteria: (i) less than 10% missing values, (ii) minor allele frequency of greater than 2.5%, (iii) no more than three heterozygous genotypes, and (iv) unique allele assignment for the 22 replicated checks of genotype B73.
Restrictions in hybrid seed production and restricted budget were the main reason for choosing an augmented design with replicated checks, but unreplicated hybrids.
Another strategy using rapid mapping to examine QTL by environment effects would be to divide the large population between environments and augment with replicated checks to adjust for the environmental effects.
Single field trials (hereafter considered as single environments) were carried out based on a modified augmented design layout with three replicated checks (by rows and columns) and unreplicated accession plots of 4 m.
Similar(54)
As Mr. Ellis sees it, the ideological and temperamental diversity of these leaders was a central element in their success: Their different beliefs about the meaning of the American Revolution created an intellectual ferment that enhanced creativity and "replicated the checks and balances of the Constitution with a human version of the same principle".
As can be seen from graphs, measurements were replicated to check repeatability (trials I-III).
Any positive results need to be double checked and replicated, preferably using a different detector (one reason why the LHC has several).
obs).. Following scoring optimization and replicate checking, all 29 replicate profiles were removed; in addition, 22 putative natural hybrid individuals identified based on morphological characters were also removed and will be discussed in detail in a separate publication (Meudt et al. unpubl. data).
Most importantly, to evaluate the potential for PCR errors and to obtain an estimate of the number of false positives, 3 different PCR products from 12 samples were sequenced and the concordance of heteroplasmic sites (identified using 0.2% as the lowest heteroplasmy threshold [ 24, 27]) between replicates checked.
Check for senescence Before a cell replicates, we check that none of its telomeres have fallen below the critical value.
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