On the other hand, the primary criticism of disc injury models is that the rapid advancement of degeneration does not replicate changes seen in human degeneration, which tends to develop over the course of many years.
Only if the average of all three replicates had value >1.5 fold and all the replicates changed in the same direction (up or down regulated) did we consider such genes to be significantly regulated by hypoxia.
The consistency of the embedment changes between the pipelines, their correlation with metocean conditions, and the ability to replicate these changes in model scale tests suggests that such changes can be accounted for in more effective pipeline design.
Importantly, the peptide-injection model appears to replicate the changes in cellular properties and brain microenvironments evident in inflamed brain in the progression of AD pathology.
The previously published changes to miRNA profiles in PD were limited to a small number of samples, and therefore it was important to replicate these changes in a larger cohort of PD brain samples.
Disease manifestation is more severe in Tg Pkd1l2)/ Tg Pkd1l2) probably because the BAC transgene does not exactly replicate the changes in Pkd1l2 gene expression caused by the ostes ENU mutation.
We have found that in GCs of young adult Ts65Dn mice (P40 60), which replicate cerebellar changes in DS (20% shrinking of cerebellar volume, 14% narrowing of the granular layer, 24% drop in GC density) (Baxter et al., 2000; Roper et al., 2006), the electrical properties of the surviving GCs are not identical to those of GCs in wild-type mice.
Since then, multiple studies have identified and replicated smoking-associated changes in DNA methylation at several genes, including the well replicated aryl hydrocarbon receptor repressor (AHRR) gene [ 47- 49] and several other regions.
Also, the lung model was not able to replicate the change in airway resistance and compliance caused by generated airway pressure.
We were unable to replicate this change in pattern beyond 50d despite testing additional 75d birds using methods designed to mimic the other study and employing the same subject number (see Methods).
