The decrease could be replicated by immunoblot analysis of bulk histones isolated under the same conditions, indicating that the decrease in H3S10P staining observed in the embryos was not due to masking of the H3S10P epitope by another molecule such as a H3S10P-binding protein (data not shown).
The obtained data were statistically analyzed as a randomized complete block design with three replicates by analysis of variance (ANOVA) using the statistical package software SAS SAS Institute Inc.., 2000, Cary, NC., USA).
Bootstrap with 1000 replicates was performed by analysis of P-distance.
Comparison of gene expression profiles between BTH- and mock-treated NB with four biological replicate experiments, followed by analysis of variance (ANOVA), identified 1,510 BTH-responsive genes (p value <0.05, >2-fold change in expression).
Bootstrap proportions were calculated by analysis of 1500 replicates.
Bootstrap proportions (BP) were calculated by analysis of 1000 replicates for NJ and by the RELL method [ 31] on the 2000 top-ranking trees for ML analyses.
Repeatability (intraday precision) of the method was determined by analysis of three replicates of three sample solutions (ethanol extracts of acetaminophen) of different concentrations (0.10, 0.20, and 0.30 mg · mL−1) under the same operating conditions over a short interval of time (the same day).
Arithmetic means of these means and variances, and harmonic means of Ne and Nb, were calculated across replicates and compared with values predicted by analysis of the vital rates, using AgeNe.
The first stage was to characterise assay performance (mean value±precision) by analysis of 16 replicates of each of three different QCs representing high-, mid- and low-range concentrations on the calibration curve.
Bootstrap support for internal branches was estimated by analysis of 1,000 pseudo replicates.
