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Nine of these 16 alleles (56%) have only a duplication (Zhang et al., 2013), which indicates that the target site was replicated at the time of RET; whereas seven alleles have an insertion, which indicates the target site was unreplicated.
Nine of these 16 alleles (56%) have only a duplication (Zhang et al., 2013), which indicates that the target site was replicated at the time of RET; whereas seven alleles have an insertion, which indicates that the target site was unreplicated.
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Specifically, improvements in gammaretroviral vector development have taken place since those initially used to conclude that gene transfer by gammaretroviral vectors occurs only in cells that are actively replicating at the time of transduction.
This result suggests that the herpesvirus was replicating at the time of death in the sampled tonsil tissue as messenger RNA (mRNA) with the herpesvirus sequence was detected.
pET30a/His-EDDIE-GFP was replicated at the same time.
p5-6C (RI = −0.14) replicated 3−4 min earlier than did a chromosomal fragment containing the early replicating origin, ARS305, whereas p5-6m7C (RI = 0.1) replicated at the same time as ARS305.
Such tighter expression regulation is also in agreement with a disproportionally low abundance of growth important genes, even in comparison to the part of the large chromosome that is replicated at the same time.
DOI: http://dx.doi.org/10.7554/eLife.11134.001 DNA replication is complicated because the two strands that form its "double helix" structure run in opposite directions and need to be replicated at the same time.
Values from this curve were used for normalization, so that each time point had a mean relative copy number equivalent to the proportion of the genome replicated at that time point.
A direct way to test the relevance of the replication model for a given organism consists in analyzing the correlation between the proportion of dCTPs and dGTPs at time t rep during the S phase and the equilibrium GC content (or the ratio R t of transition rates) of the sequences replicated at that time.
Based on our comparison of replication timing in fibroblast and lymphoblastoid cells, these regions are more likely to replicate at the same time in multiple cell types than sequences that replicate at other times in the S phase.
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CEO of Professional Science Editing for Scientists @ prosciediting.com