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pET30a/His-EDDIE-GFP was replicated at the same time.
p5-6C (RI = −0.14) replicated 3−4 min earlier than did a chromosomal fragment containing the early replicating origin, ARS305, whereas p5-6m7C (RI = 0.1) replicated at the same time as ARS305.
Such tighter expression regulation is also in agreement with a disproportionally low abundance of growth important genes, even in comparison to the part of the large chromosome that is replicated at the same time.
DOI: http://dx.doi.org/10.7554/eLife.11134.001 DNA replication is complicated because the two strands that form its "double helix" structure run in opposite directions and need to be replicated at the same time.
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Based on our comparison of replication timing in fibroblast and lymphoblastoid cells, these regions are more likely to replicate at the same time in multiple cell types than sequences that replicate at other times in the S phase.
Furthermore we could show that the viruses can replicate at the same time in the cells.
The absence of correlation could be due to the fact that all origins do not fire simultaneously and therefore two loci replicating at the same time could be located at very different distances from their respective active origins.
Recent work suggests that the pattern of the SNCAs in cancer depends on the spatial proximity of DNA regions replicating at the same time, and thus is a consequence of the fractal globule configuration [151].
This allowed us to evaluate regions with overlapping clones that are similar in size to an average replicon and therefore should replicate at the same time in S phase.
If the bias determinant were causing a delay in the activation of ARS1 N, then deletion of the bias determinant should have no effect on the time of activation of ARS1 S and the two plasmids should replicate at the same time.
Note that both donor mating-type cassettes, HML and HMR, were replicated at approximately the same time and before the mating-type locus MAT.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com