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Flp1- FRT recombination produces equal amount of two structural isomers (Fig. 1C) and can initiate DRCR if Flp1- FRT recombination occurs just after either FRT site is replicated, as shown in Fig. 1D.
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In YE-PC8-infected host cells treated with lovastatin, YE-PC8 failed to replicate as shown by low viral copy-number and low luciferase activity.
This can be verified by simulating 5000 replicates as shown in Figure 3.
Achieving a confidence interval of ±10 ms now only requires 11 25 replicates, as shown in Table 4, which might be achievable.
The results of the PCA plot showed consistency across biological replicates, as shown in Figure 2. The first two principal components (PC1 and PC2) explained 77.5percentt of the total variability in gene expression (62.2 percent and 15.3percentt respectively).
The experiment revealed 113 proteins, of which 87% were replicated 4 times, as shown in Figure 1B and Table 1.
Changes in expression levels of these 3 genes are successfully replicated using qPCR as shown in the Fig. 2, right panel.
These figures are important to replicate as they show that Cav1 expression in fibroblasts contributes to remodeling of the tumor microenvironment in vivo, and that this remodeling correlates with the amount of metastasis.
Semi-quantitative RT-PCR was performed in each of two independent biological replicate experiments as shown in Figure 4A.
The basic idea is to replicate the nodes as shown in Figure 2(b).
We checked that NK cells were not able to replicate HIV-1, as shown by the lack of p24 detection in culture supernatant and the lack of intracellular p24 staining (detected by FACS) in NK cells (data not shown).
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