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The average recovery and relative standard deviation for six replicated analysis were 101.0% and 2.77%, respectively.
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The limitations and advantages of the new method compared to replicate analysis are presented.
In order to investigate intra-day accuracy and precision; DOX-plasma-samples replicate analysis was performed whereby six replicate samples from LLOQC, LQC, MQC, and HQC as well as their calibration curve were selected in the run.
The coefficients of variation of replicate analysis were determined for precision of analysis; the variations were found to be less than 10%.
The protein abundances from each replicate analysis were used as the primary variables and 10 patient samples were considered as primary observations.
Each replicate analysis was carried out on 3 separate days.
The MIDAS in-slide replicate analysis was applied to merge replicates of each gene.
If replicate analysis is not possible (e.g, limited sample volume), another alternative is including additional QCBs and QCs within the analytical batch.
The limit of detection (LOD) of the passive monitor was < 0.01 μg, and the coefficient of variability for replicate analysis was 0.11 (Hammond and Leaderer 1987).
The intra-sample variation in replicate analysis was higher for mRNA ratios than for DNA ratios, possibly owing to RNA stability.
In order to study the pathological effects of the infection in the organs where BTV replicates, histological analysis were performed on material of several organs extracted from BTV infected and uninfected IFNAR mice at 48 h.p.i.
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