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Gene expression profiling of liver and adipose tissue samples from the same rats investigated in the miRNA experiment was performed using Sentrix® BeadChip RatRef-12 v1 Whole-Genome Gene Expression Arrays (Illumina Inc., San Diego, California, USA), which contain 22,523 oligonucleotides probes (replicated an average of 30 times).
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To transform the 16S rRNA tree replicates into the supertree replicates requires an average of 14 elementary operations and to convert the 16S rRNA replicates into the SHOT variant 1 replicates is 18.
The similar IncX1 plasmid R485 has previously been reported to replicate at an average copy number of 3 5 plasmid copies per E. coli cell [24] indicating that the pX1.0 replicon operates at levels comparable to its natural equivalents.
Using these optimal factor values under experimental conditions in three independent replicates, an average content of 97.8 ± 0.6% was achieved, and this value was well within the range predicted by the model.
For each biological replicate, an average Ct value was calculated from the two technical replicates.
This tree shape is reflected in low levels of standing diversity; across replicates, an average of 5.68 years of evolution separates two randomly sampled viruses in the population.
For example, in the consecutive sampling of duplicate hybridizations and biological culture replicates, an average 6.8% and 5.0% of the samples failed the Kolmogorov-Smirnov test at the level of 0.05.
An evaluation of the variance between the biological replicates revealed an average standard deviation across all replicates of less than 6% for biomass concentration and remaining phosphate levels in the growth medium, and less than 10% for the production levels of undecylprodigiosin and actinorhodin.
A high correlation was observed between replicates, indicating a high degree of reproducibility of technical and biological replicates, with an average Pearson's correlation coefficient of r = 0.898 for the procyclic form replicates and r = 0.843 for the bloodstream form replicates following log transformation.
This threshold exceeds the 15.7% average increase in DNA quantities observed between GQ technical replicates, with an average coefficient of variation of 12.3% (n = 6 pairs of replicates).
This process was repeated for 100 different random replicates and an average and standard deviation for each coordinate was recorded.
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