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Actors from Germany performed the play-acted stimuli (42 actors each replicated a maximum of three recordings of equivalent emotional content).
Play-acted stimuli were performed by actors from Berlin, Hanover, and Göttingen, in Germany (each replicated a maximum of three recordings of equivalent emotional content; total of 42 actors).
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A heuristic search with tree bisection-reconnection (TBR) branch swapping and 100 additional sequence replicates, saving a maximum of 100 trees per replicate, was used for the MP tree search, and a distance measure under the maximum likelihood settings was used for the NJ tree search.
An initial heuristic search was carried out with 1000 replicates, holding a maximum of 10 trees per replicate.
One hundred jackknife replicates were performed with one TBR search per replicate and a maximum of 100 trees held per TBR search.
One thousand replicates were performed with 10 TBR searches per replicate and a maximum of 100 trees held per TBR search.
Each sample was analysed at least in duplicate and a maximum of 10% difference between replicate analyses was accepted.
For the BG model fit to data simulated under an ancient exponential population decline (NEGG model, A parameterization) with n = 50, convergence required an average of more than 323 optimizations per replicate (up to a maximum of 1011 optimizations).
When individual strains were examined in replicate, we found that a maximum of three gene target differences may occur between replicates.
Mean values of duplicate measurements of one culture are given; replicate values deviated by a maximum of ± 1 ‰. a two isomers, separated by retention time.
On each plate, every sample was replicated across four neighbouring wells, resulting in a maximum of 96 samples per plate.
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