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Each analysis included four simultaneous and incrementally heated Markov chains; each replicate used default heating values.
Dorsal regions of the rostral and caudal slices adopted an earlier phase, which is similar to that observed in the LD 12∶12 replicate used for immunohistochemistry and indicates that the relative earliness of dorsal regions is likely influenced by a factor other than entrainment condition.
Reactions (in triplicate for each biological replicate) used RT2 qPCR Mastermix (SABiosciences, Frederick, MD, USA), 200 nM forward and reverse primers, 200 uM dNTPs, and 1 uL cDNA diluted 1∶5 or 1∶10, using the Rotorgene RG-3000 thermoCorbett ResearchResearch, Kirkland, PQ, Canada).
Each replicate used a fraction of leaf mixture for RNA extraction.
The number of replicates in the vertebrate predator experiment was limited by the number of available Daphnia and each replicate used a novel predator.
Additionally, specific primers flanking the 70-mer oligo used in the array were designed and used in a qRT-PCR reaction (see Methods) starting with total RNA from the same biological replicate used for the RNA blot hybridization analysis.
Similar(50)
Or when single-celled organisms began to replicate using DNA?
Furthermore, the porosity of natural calciferous shells may be difficult to replicate using a fabricator.
Her style is easy to replicate using items you can actually re-use after Halloween.
Experiments to test quenching efficiency were performed in replicate using Escherichia coli lysate.
For these daily measurements, one extra replicate was used in each test concentration that was treated the same as the replicates used for determination of the algal cells.
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CEO of Professional Science Editing for Scientists @ prosciediting.com