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Furthermore, two distinct population of cells: one is active population able to replicate, Type I cell, and another is stalled population unable to replicate due to toxicity of inhibitors, Type II cell, can also be included in the cell growth model (Wang et al. 2014).
Additional file 2: Figures S2A and B show the pairwise correlation plots within each replicate type and cell line.
The important attributes describing the overall study, such as disease type, study type, and replicate type, are not available through the NCBI interface or repositories.
In MetaRNA-Seq, a RNA-Seq study can be annotated for study type, disease category, sample type, replicate type, and custom annotation.
The combination of anorganic bone mineral (ABM/P-15) and synthetic peptide P-15 acts as a bioactive attachment factor that aims to replicate type I collagen binding mechanism with osteogenic precursor cells in bone tissue [ 11, 12].
Moreover, project- and study-level metadata lack manual or automatic curation by categories, such as disease type, time series, case-control, or replicate type, which are vital to comprehending any RNA-Seq study.
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This allows for a comparison on both replicate types.
Replicate typing revealed that clinical isolates and environmental isolates contain similar plasmid types.
As only one individual was heterozygote, it is not possible to calculate an allelic dropout rate, but from the four pyrosequencing results from the heterozygote individual, two replicate typing events expressed allelic dropout.
It is interesting to note that TCF4, a transcription factor, coded by TCF7L2, the most strongly associated and replicated type 2 diabetes susceptibility loci, is an integral part of Wnt-1 signaling [23] [29].
Transcription factor 7-like 2 gene (TCF7L2) rs7903146 to this date remains the strongest and most widely replicated type 2 diabetes susceptibility locus [ 1, 2].
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